ABSTRACT:

Four bacterial isolates from four different environmental samples (agricultural soil, compost soil and sewage sluclge- anaerobic and aerobic wastewaters) were screened for their ability to mineralize 2-methoxyethanol under aerobic conditions. Isolate from anaerobic sew;1ge wastewater was most efficient with an average of 48.0rnmol/day mineralisation capacity. Morphological, physiological, biochemical and molecular characterization of the isolate showed that the strain, designated VB is of the genus Pseudomonas. 16S rRNA sequence analysis sh,.:iwed that the organism is related to Pseudomonas putida at 99.9°.t and Pseudomonas plecog/ossicida at 99.8% similarity level. It is related to members of the genus Pseudomomas belonging to the rRNA group 1 within the gamma M Proteobacteria. The G+C, content 64.5±0.8mol% is within the range characteristic of the genus Pseudomonas.